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Updated: Jun 4, 2026
06:21
Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry
Published on: July 12, 2013
Desorption Electrospray Ionization: Proteomics Studies by a Method That Bridges ESI and MALDI.
CSH Protocols
|March 2, 2011
Summary
Desorption electrospray ionization (DESI) analyzes surface materials using charged droplets. This ambient ionization technique is versatile for various sample types, including biological tissues.
Area of Science:
- Analytical Chemistry
- Surface Science
Background:
- Desorption electrospray ionization (DESI) is a desorption ionization technique.
- It shares similarities with matrix-assisted laser desorption/ionization (MALDI) and electrospray ionization (ESI).
- Unlike ESI, DESI analyzes analytes directly from surfaces without prior dissolution.
Purpose of the Study:
- To describe the principles and applications of DESI.
- To highlight its utility in analyzing diverse surface-bound materials.
Main Methods:
- Utilizes a microelectrospray ion source to generate charged droplets or ions.
- Applies a high electrical potential (kilovolts) to the spray solution.
- Employs pneumatic nebulization for desolvation and directs the spray at the sample surface in an ambient environment.
Main Results:
- Ionization of surface molecules occurs upon impact with charged particles.
- DESI is effective for analyzing deposited samples and natural surfaces like biological tissues and minerals.
Conclusions:
- DESI is a powerful ambient ionization technique for surface analysis.
- Its ability to analyze samples in situ makes it valuable for various scientific fields.
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