Estimation of Free Thiols and Disulfide Bonds Using Ellman's Reagent
CSH Protocols
|March 2, 2011
Abstract:
INTRODUCTIONThis protocol describes a method for quantitating the amount of free thiols and disulfide bond linkages in proteins. It uses the sulfhydryl reagent 5,5'-dithiobis (2-nitrobenzoic acid), or DTNB, which is also referred to as Ellman's reagent.
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