Streamlined protocol for antibody staining of large numbers of samples in Drosophila

INTRODUCTIONThe most common reason for generating antibodies against a particular protein in Drosophila is to examine its pattern of endogenous expression during development. Thus, once an antibody is shown to stain transfected cells, the next step is to try staining Drosophila tissues, usually embryos (described here), imaginal discs, or ovaries. This streamlined protocol can be used for processing a large number of samples, e.g., screening monoclonal antibodies.