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Denaturing protein immunoprecipitation from Mammalian cells

William P Tansey1

  • 1Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.

CSH Protocols
|March 2, 2011
PubMed

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Immunoprecipitation01:20

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...