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Immunoprecipitation with an Anti-Epitope Tag Affinity Gel to Study Protein-Protein Interactions
Published on: January 5, 2024
Nondenaturing protein immunoprecipitation from Mammalian cells
1Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.
CSH Protocols
|March 2, 2011
Abstract:
INTRODUCTIONThis protocol describes a nondenaturing immunoprecipitation (IP) for mammalian cells. Where possible, we prefer to use denaturing IPs to recover labeled proteins from pulse-chase experiments. However, the nondenaturing protocol is useful when one wishes to separate soluble from insoluble proteins, or when the antibody being used recognizes a native epitope.
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