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Updated: Jun 4, 2026
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Fixation of Embryonic Mouse Tissue for Cytoneme Analysis
Published on: June 16, 2022
Fixation of mouse embryos and tissues
CSH Protocols
|March 2, 2011
Summary
This protocol details tissue fixation methods for mouse embryos and tissues using paraformaldehyde, Bouin's fixative, or methanol/DMSO. These techniques ensure proper sample preservation for downstream biological analyses.
Area of Science:
- Developmental Biology
- Histology
Background:
- Accurate preservation of biological samples is crucial for subsequent analyses.
- Various fixation methods exist, each with specific applications and limitations.
Purpose of the Study:
- To provide a standardized protocol for fixing mouse embryos and tissues.
- To offer reliable methods using common fixatives like paraformaldehyde, Bouin's, and methanol/DMSO.
Main Methods:
- Detailed procedures for paraformaldehyde fixation.
- Protocols for using Bouin's fixative.
- Methods for methanol/DMSO solution fixation.
Main Results:
- Established protocols for effective fixation of mouse embryonic and tissue samples.
- Demonstrated applicability of three distinct fixation techniques.
Conclusions:
- The presented methods offer versatile options for researchers.
- These protocols facilitate consistent and high-quality sample preparation for diverse studies.
