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Equipment Setup for Fluorescence Imaging with One-Nanometer Accuracy (FIONA)
Paul R Selvin1, Tyler Lougheed, Melinda Tonks Hoffman
1Biophysics Center and Physics Department, University of Illinois, Urbana, IL 61801, USA.
CSH Protocols
|March 2, 2011
Abstract:
INTRODUCTIONFIONA, short for fluorescence imaging with one-nanometer accuracy, is a simple method for achieving localization of single (or single groups of) fluorophores with nanometer accuracy in the xy plane. This article describes procedures for setting up the equipment necessary for FIONA and achieving total internal reflection (TIR).
