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Fluorescence Imaging with One-nanometer Accuracy (FIONA)
Published on: September 26, 2014
Data Analysis Methods for Fluorescence Imaging with One-Nanometer Accuracy (FIONA)
Paul R Selvin1, Tyler Lougheed, Melinda Tonks Hoffman
1Biophysics Center and Physics Department, University of Illinois, Urbana, IL, 61801, USA.
CSH Protocols
|March 2, 2011
Abstract:
INTRODUCTIONFIONA, short for fluorescence imaging with one-nanometer accuracy, is a simple method for achieving localization of single (or single groups of) fluorophores with nanometer accuracy in the xy plane. Data analysis of a FIONA experiment requires the use of several software programs. Data must be acquired and exported into a proper format before analysis can take place. This article describes various options for data analysis.
