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Related Experiment Video

Updated: Jun 4, 2026

Fluorescence Imaging with One-nanometer Accuracy (FIONA)
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Fluorescence Imaging with One-nanometer Accuracy (FIONA)

Published on: September 26, 2014

Data Analysis Methods for Fluorescence Imaging with One-Nanometer Accuracy (FIONA).

Paul R Selvin1, Tyler Lougheed, Melinda Tonks Hoffman

  • 1Biophysics Center and Physics Department, University of Illinois, Urbana, IL, 61801, USA.

CSH Protocols
|March 2, 2011
PubMed
Summary
This summary is machine-generated.

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Fluorescence Imaging with One-nanometer Accuracy (FIONA) enables precise localization of single fluorophores. This article details software options for analyzing FIONA experimental data.

Area of Science:

  • Biophysics
  • Optical Microscopy
  • Nanotechnology

Background:

  • Single-molecule localization microscopy is crucial for understanding biological processes at the nanoscale.
  • Accurate localization of fluorophores is essential for high-resolution imaging.
  • Existing methods may have limitations in accuracy or complexity.

Purpose of the Study:

  • To provide an overview of data analysis options for Fluorescence Imaging with One-nanometer Accuracy (FIONA) experiments.
  • To guide researchers in selecting appropriate software for FIONA data processing.
  • To facilitate the accurate interpretation of nanometer-scale localization data.

Main Methods:

  • Description of various software tools and workflows for FIONA data analysis.
  • Explanation of data acquisition and export requirements for analysis.

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Related Experiment Videos

Last Updated: Jun 4, 2026

Fluorescence Imaging with One-nanometer Accuracy (FIONA)
11:56

Fluorescence Imaging with One-nanometer Accuracy (FIONA)

Published on: September 26, 2014

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes
08:26

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes

Published on: November 23, 2021

A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules
10:45

A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules

Published on: June 20, 2020

  • Discussion of different analytical approaches for fluorophore localization.

    • Main Results:

    • Identification of multiple software solutions suitable for FIONA data analysis.
    • Outlining of the steps involved in processing FIONA data from acquisition to analysis.
    • Highlighting the importance of proper data formatting for accurate results.

    Conclusions:

    • Effective data analysis is critical for achieving nanometer accuracy with FIONA.
    • A range of software options are available to support FIONA experiments.
    • Researchers can select the best analysis tools based on their specific experimental needs and technical expertise.