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Related Experiment Video

Updated: Jun 4, 2026

Use of Synaptic Zinc Histochemistry to Reveal Different Regions and Laminae in the Developing and Adult Brain
09:50

Use of Synaptic Zinc Histochemistry to Reveal Different Regions and Laminae in the Developing and Adult Brain

Published on: October 29, 2017

Imaging zinc in brain slices.

Alan R Kay

    CSH Protocols
    |March 2, 2011
    PubMed
    Summary
    This summary is machine-generated.

    This protocol details methods for visualizing zinc (Zn2+) in brain tissue. It offers practical guidance for imaging zinc within synaptic vesicles and the extracellular space using live preparations.

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    Last Updated: Jun 4, 2026

    Use of Synaptic Zinc Histochemistry to Reveal Different Regions and Laminae in the Developing and Adult Brain
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    Published on: October 29, 2017

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    Imaging Metals in Brain Tissue by Laser Ablation - Inductively Coupled Plasma - Mass Spectrometry (LA-ICP-MS)
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    Imaging Metals in Brain Tissue by Laser Ablation - Inductively Coupled Plasma - Mass Spectrometry (LA-ICP-MS)

    Published on: January 22, 2017

    Area of Science:

    • Neuroscience
    • Biochemistry
    • Cell Biology

    Background:

    • Exchangeable zinc ions (Zn2+) are concentrated in synaptic vesicles of certain glutamatergic terminals.
    • Traditional methods like Timm's stain reveal these zinc-rich terminals.
    • Recent advancements include fluorimetric probes for detecting Zn2+ in live biological preparations.

    Purpose of the Study:

    • To provide a practical protocol for imaging zinc within synaptic vesicles.
    • To offer a method for detecting zinc in the extracellular space of brain slices.
    • To enable visualization of Zn2+ in live preparations.

    Main Methods:

    • Describes two distinct methods for Zn2+ detection.
    • One method visualizes Zn2+ within synaptic vesicles.
    • The second method detects extracellular Zn2+.

    Main Results:

    • Successful visualization of Zn2+ in synaptic vesicles.
    • Successful detection of Zn2+ in the extracellular space.
    • Demonstrates applicability to tissues with high vesicular Zn2+ and extracellular Zn2+ associated with macromolecules.

    Conclusions:

    • The protocol offers practical, accessible methods for imaging zinc in neural tissue.
    • These techniques are valuable for studying the role of zinc in synaptic function.
    • Applicable to various tissues with specific zinc distribution patterns.