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Related Concept Videos

Transgenic Organisms00:53

Transgenic Organisms

Overview
DNA-only Transposons02:57

DNA-only Transposons

DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...

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Related Experiment Video

Updated: Jun 4, 2026

Generation of Transgenic Rats using a Lentiviral Vector Approach
09:07

Generation of Transgenic Rats using a Lentiviral Vector Approach

Published on: May 17, 2020

Transposon-mediated transgenesis in rats.

Chuan-Wei Jang1, Richard R Behringer

  • 1Program in Developmental Biology, Baylor College of Medicine, Houston, TX 77030, USA.

CSH Protocols
|March 2, 2011
PubMed
Summary

This study introduces an efficient method for creating transgenic rats using PiggyBac transposon technology. This novel technique significantly improves the success rate of generating genetically modified rats compared to traditional methods.

Area of Science:

  • Genetics
  • Molecular Biology
  • Animal Models

Background:

  • Generating transgenic rats is crucial for biomedical research.
  • Traditional methods like linear DNA microinjection have limited efficiency.

Purpose of the Study:

  • To describe an efficient protocol for producing transgenic rats.
  • To present an alternative to traditional transgenic rat generation methods.

Main Methods:

  • Coinjection of PiggyBac transposon plasmid DNA and transposase mRNA into fertilized rat eggs.
  • Microinjection of pronuclei followed by immediate transfer to pseudopregnant foster females.

Main Results:

  • Achieved transformation frequencies ranging from 33% to 100% (average ~80%).

More Related Videos

Generation of Topically Transgenic Rats by In utero Electroporation and In vivo Bioluminescence Screening
08:44

Generation of Topically Transgenic Rats by In utero Electroporation and In vivo Bioluminescence Screening

Published on: September 24, 2013

Lentiviral Mediated Production of Transgenic Mice: A Simple and Highly Efficient Method for Direct Study of Founders
10:21

Lentiviral Mediated Production of Transgenic Mice: A Simple and Highly Efficient Method for Direct Study of Founders

Published on: October 7, 2018

Related Experiment Videos

Last Updated: Jun 4, 2026

Generation of Transgenic Rats using a Lentiviral Vector Approach
09:07

Generation of Transgenic Rats using a Lentiviral Vector Approach

Published on: May 17, 2020

Generation of Topically Transgenic Rats by In utero Electroporation and In vivo Bioluminescence Screening
08:44

Generation of Topically Transgenic Rats by In utero Electroporation and In vivo Bioluminescence Screening

Published on: September 24, 2013

Lentiviral Mediated Production of Transgenic Mice: A Simple and Highly Efficient Method for Direct Study of Founders
10:21

Lentiviral Mediated Production of Transgenic Mice: A Simple and Highly Efficient Method for Direct Study of Founders

Published on: October 7, 2018

  • Demonstrated significantly higher efficiency compared to traditional methods (3%-41% founders).

    • Conclusions:

    • Transposon-mediated transformation is a highly efficient method for generating transgenic rats.
    • This protocol offers a superior alternative for creating genetically modified rat models.