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Updated: Jun 4, 2026
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Whole Mount in Situ Hybridization of E8.5 to E11.5 Mouse Embryos
Published on: October 10, 2011
Imaging mouse embryos after whole-mount in situ hybridization
CSH Protocols
|March 2, 2011
Abstract:
INTRODUCTIONEmbryos that are processed for whole-mount in situ hybridization can be photodocumented after the staining procedure depending on the region of the embryo of interest. The embryos can be photodocumented immediately after the color reaction or after clearing in glycerol solutions. This protocol describes methods for embryo clearing and imaging.
