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Related Experiment Video

Updated: Jun 4, 2026

Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos
11:25

Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos

Published on: February 22, 2016

Handling mouse blastocysts for fixation.

Andras Nagy, Marina Gertsenstein, Kristina Vintersten

    CSH Protocols
    |March 2, 2011
    PubMed
    Summary
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    This protocol details histological techniques for whole or sectioned blastocysts. Transferring embryos to the oviduct simplifies handling for histological studies like in situ hybridization and immunohistochemistry.

    Area of Science:

    • Developmental Biology
    • Histology
    • Embryology

    Background:

    • Whole blastocysts or sections are used for histological studies.
    • Techniques differ for whole versus sectioned embryos.
    • Histological analysis is crucial for understanding embryonic development.

    Purpose of the Study:

    • To describe a protocol for histological studies of blastocysts.
    • To present optimized handling techniques for whole and sectioned embryos.
    • To facilitate in situ hybridization and immunohistochemistry on blastocysts.

    Main Methods:

    • Fixation of whole blastocysts or sections.
    • Transfer of blastocysts into the oviduct for sectioning.
    • Histological processing of oviduct-mounted embryos.

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    Fixation of Embryonic Mouse Tissue for Cytoneme Analysis
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    Fixation of Embryonic Mouse Tissue for Cytoneme Analysis

    Published on: June 16, 2022

    Whole Mount in Situ Hybridization of E8.5 to E11.5 Mouse Embryos
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    Whole Mount in Situ Hybridization of E8.5 to E11.5 Mouse Embryos

    Published on: October 10, 2011

    Related Experiment Videos

    Last Updated: Jun 4, 2026

    Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos
    11:25

    Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos

    Published on: February 22, 2016

    Fixation of Embryonic Mouse Tissue for Cytoneme Analysis
    08:46

    Fixation of Embryonic Mouse Tissue for Cytoneme Analysis

    Published on: June 16, 2022

    Whole Mount in Situ Hybridization of E8.5 to E11.5 Mouse Embryos
    13:54

    Whole Mount in Situ Hybridization of E8.5 to E11.5 Mouse Embryos

    Published on: October 10, 2011

    Main Results:

    • The oviduct serves as an effective carrier for sectioned embryos.
    • This method simplifies handling during histological processing.
    • Successful application of in situ hybridization and immunohistochemistry is implied.

    Conclusions:

    • A simplified protocol for blastocyst histology is presented.
    • Oviduct transfer enhances embryo handling for sectioning.
    • This method supports advanced histological analyses of early embryos.