Handling mouse blastocysts for fixation

INTRODUCTIONWhole blastocysts or sections of blastocysts can be fixed and used for histological studies, including in situ hybridization and immunohistochemistry. As described in this protocol, different handling techniques are required for whole versus sectioned embryos. For sectioning, it is more convenient to transfer blastocysts into the ampulla of an oviduct prior to fixation. The oviduct serves as a carrier that is easy to handle during subsequent processing for histology.