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Updated: Jun 4, 2026

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
12:20

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Published on: August 6, 2014

Imaging real-time gene expression in living systems with single-transcript resolution: image analysis of single mRNA

Amber L Wells1, John S Condeelis, Robert H Singer

  • 1Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.

CSH Protocols
|March 2, 2011
PubMed
Summary

This study details a method for quantifying single green fluorescent protein (GFP) molecules in cells using fluorescence microscopy. The protocol establishes a GFP calibration curve for accurate single-molecule detection and quantification in fixed and live cells.

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