Fixation of imaginal discs in Drosophila

INTRODUCTIONImaginal disc primordia appear in Drosophila embryos as clusters of cells that invaginate from the embryonic epithelium. During metamorphosis, the imaginal discs form much of the outer covering of the developing adult. Generation of genetic mosaics is useful for removing or adding gene function to imaginal cells. This protocol describes the methods for fixing such tissues in preparation for immunohistological techniques to monitor gene expression.