Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Blood biomarker outliers and clinical events during intensive care in patients with traumatic brain injury.

Brain & spine·2026
Same author

Association of acute blood biomarkers with diffusion tensor imaging and outcome in patients with traumatic brain injury presenting with GCS of 13-15.

NeuroImage. Clinical·2026
Same author

Evaluation of the prehospital diagnostic accuracy of a novel point-of-care test for NT-proBNP, D-dimer and H-FABP and large-vessel occlusion risk assessment (LVOCheck-EVA): a protocol for a multicenter prospective observational study in patients suspected of having a stroke.

Frontiers in neurology·2025
Same author

IL6 in Combination with Either NfL, NTproBNP, or GFAP to Safely Discharge Children with Mild Traumatic Brain Injury.

Journal of neurotrauma·2025
Same author

Cerebrospinal Fluid-Derived Extracellular Vesicles: A Proteomic and Transcriptomic Comparative Analysis of Enrichment Protocols.

Journal of extracellular biology·2025
Same author

Proteomic analysis of plasma-derived extracellular vesicles: Insights into acute stroke pathophysiology.

Journal of proteomics·2025

Related Experiment Video

Updated: Jun 4, 2026

Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools
07:01

Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools

Published on: August 19, 2025

Analysis of proteomes using the molecular scanner.

Catherine Déon, Willy Bienvenut, Jean-Charles Sanchez

    CSH Protocols
    |March 2, 2011
    PubMed
    Summary

    A novel molecular scanner visualizes proteomes using 2D gel electrophoresis and mass spectrometry. This method simplifies sample handling for direct protein identification from membranes, creating detailed multidimensional proteome maps.

    More Related Videos

    A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions
    09:00

    A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions

    Published on: April 18, 2025

    Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples
    14:51

    Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples

    Published on: November 13, 2021

    Related Experiment Videos

    Last Updated: Jun 4, 2026

    Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools
    07:01

    Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools

    Published on: August 19, 2025

    A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions
    09:00

    A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions

    Published on: April 18, 2025

    Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples
    14:51

    Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples

    Published on: November 13, 2021

    Area of Science:

    • Proteomics
    • Analytical Chemistry
    • Biotechnology

    Background:

    • 2D gel electrophoresis is a key technique for protein separation.
    • Direct protein identification from gels or membranes remains challenging.
    • Minimizing sample handling is crucial for mass spectrometry analysis.

    Purpose of the Study:

    • To introduce a molecular scanner for enhanced proteome visualization.
    • To enable direct protein identification from PVDF membranes.
    • To create multidimensional proteome maps with reduced sample handling.

    Main Methods:

    • Proteins are separated by 2D gel electrophoresis and simultaneously digested during electrotransfer.
    • Peptide mass fingerprint (PMF) analysis is performed using MALDI-TOF-MS.
    • Dedicated software tools are used for data processing, visualization, and map creation.

    Main Results:

    • The molecular scanner allows for direct protein identification from PVDF membranes.
    • Sample handling is minimized, requiring only small sample sizes (micrometer scale).
    • Multidimensional proteome maps can be generated for comprehensive visualization.

    Conclusions:

    • The molecular scanner is a powerful tool for flexible and annotated 2D gel electrophoresis map creation.
    • This method streamlines the proteomic workflow from separation to identification.
    • It offers a significant advancement in visualizing and analyzing complex proteomes.