Protein arrays: preparation of microscope slides

INTRODUCTIONThis protocol describes the preparation of glass microscope slides with two particularly useful substrates: aldehyde and BSA-NHS. On aldehyde slides, amines on the proteins form a Schiff base with the aldehydes on the slide. BSA-NHS (bovine serum albumin-N-hydroxysuccinimide) slides contain a layer of BSA molecules that are chemically activated for covalent attachment of proteins to the slide. In this case, amines on the proteins of interest form amide or urea bonds with the activated carboxylates or activated amines of the BSA, respectively.