Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

HIF regulates multiple translated endogenous retroviruses: Implications for cancer immunotherapy.

Cell·2025
Same author

A call to adapt the regulation of HLA testing for T cell receptor-based therapeutics.

Nature reviews. Drug discovery·2023
Same author

Tissue-resident memory and circulating T cells are early responders to pre-surgical cancer immunotherapy.

Cell·2022
Same author

Phenotype, specificity and avidity of antitumour CD8<sup>+</sup> T cells in melanoma.

Nature·2021
Same author

Phase 1 dose escalation study of seribantumab (MM-121), an anti-HER3 monoclonal antibody, in patients with advanced solid tumors.

Investigational new drugs·2021
Same author

Unbiased Screens Show CD8<sup>+</sup> T Cells of COVID-19 Patients Recognize Shared Epitopes in SARS-CoV-2 that Largely Reside outside the Spike Protein.

Immunity·2020

Related Experiment Video

Updated: Jun 4, 2026

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions
08:07

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Published on: August 2, 2015

Protein arrays: sandwich approach for analyzing complex solutions.

Gavin Macbeath

    CSH Protocols
    |March 2, 2011
    PubMed
    Summary

    This protocol details a sandwich assay for protein arrays, enabling quantification of protein abundance and modification states without direct protein labeling. This indirect detection method enhances specificity by using two distinct antibodies for each target protein.

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Assay Development

    Background:

    • Protein arrays are crucial for quantifying protein abundance and modifications in complex biological samples.
    • Accurate protein quantification requires specific capture and detection methods.
    • Existing methods may involve direct labeling of target proteins, which can be complex.

    Purpose of the Study:

    • To present a detailed protocol for an indirect labeling method in protein arrays.
    • To enable sensitive and specific protein detection without labeling the proteins of interest.
    • To outline the advantages and challenges of the sandwich assay approach for protein quantification.

    Main Methods:

    • Immobilization of capture reagents (e.g., antibodies) onto slides.

    More Related Videos

    Identifying Protein-protein Interaction Sites Using Peptide Arrays
    07:44

    Identifying Protein-protein Interaction Sites Using Peptide Arrays

    Published on: November 18, 2014

    A Guided Materials Screening Approach for Developing Quantitative Sol-gel Derived Protein Microarrays
    10:44

    A Guided Materials Screening Approach for Developing Quantitative Sol-gel Derived Protein Microarrays

    Published on: August 26, 2013

    Related Experiment Videos

    Last Updated: Jun 4, 2026

    Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions
    08:07

    Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

    Published on: August 2, 2015

    Identifying Protein-protein Interaction Sites Using Peptide Arrays
    07:44

    Identifying Protein-protein Interaction Sites Using Peptide Arrays

    Published on: November 18, 2014

    A Guided Materials Screening Approach for Developing Quantitative Sol-gel Derived Protein Microarrays
    10:44

    A Guided Materials Screening Approach for Developing Quantitative Sol-gel Derived Protein Microarrays

    Published on: August 26, 2013

  • Specific capture of target antigens from solution using immobilized reagents.
  • Detection of captured antigens via a secondary, labeled antibody that binds to a different epitope.
  • Utilizing a sandwich assay format with two distinct antibodies for enhanced specificity.

    • Main Results:

    • The indirect labeling method allows for protein quantification without the need for direct protein labeling.
    • The sandwich approach enhances specificity by employing two different antibodies per protein.
    • A key challenge identified is the difficulty in assembling well-matched antibody pairs for each target protein.

    Conclusions:

    • The described sandwich assay protocol provides a robust method for protein quantification in complex mixtures.
    • This indirect detection strategy offers advantages in specificity and avoids direct protein labeling.
    • Careful selection of antibody pairs is critical for the successful implementation of this protein array technique.