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Updated: Jun 4, 2026
10:55
Staining Proteins in Gels
Published on: July 8, 2008
Summary
This protocol details the standard Coomassie Brilliant Blue R250 (CBR-250) staining method for protein visualization in SDS-PAGE gels. It also provides a simple technique for preserving stained gels for extended periods.
Area of Science:
- Biochemistry
- Molecular Biology
- Proteomics
Background:
- SDS-PAGE is a fundamental technique for protein separation.
- Coomassie Brilliant Blue R250 (CBR-250) is the most common dye for protein visualization due to its sensitivity.
Purpose of the Study:
- To describe the standard CBR-250 staining protocol for SDS-PAGE gels.
- To present a simple method for long-term storage of stained gels.
Main Methods:
- Standard Coomassie Brilliant Blue R250 (CBR-250) staining procedure.
- A straightforward protocol for preparing stained gels for archival purposes.
Main Results:
- Successful visualization of proteins in SDS-PAGE gels using CBR-250.
- Demonstration of a reliable method for long-term stained gel preservation.
Conclusions:
- The described protocol provides a robust method for protein staining and long-term storage of SDS-PAGE gels.
- This standardized approach ensures consistent and reproducible protein visualization and archival quality.
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