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Related Experiment Video

Updated: Jun 4, 2026

Tracing Gene Expression Through Detection of β-galactosidase Activity in Whole Mouse Embryos
08:42

Tracing Gene Expression Through Detection of β-galactosidase Activity in Whole Mouse Embryos

Published on: June 26, 2018

Imaging X-gal-Stained Mouse Embryos.

Akio Kobayashi

    CSH Protocols
    |March 2, 2011
    PubMed
    Summary
    This summary is machine-generated.

    This study details a method for visualizing whole mouse embryos using beta-galactosidase (lacZ) staining and microscopy. The technique allows for detailed examination of embryonic development and gene expression patterns.

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    Area of Science:

    • Developmental Biology
    • Genetics
    • Microscopy

    Background:

    • Beta-galactosidase (lacZ) staining is a common technique to visualize gene expression.
    • Whole mouse embryos are frequently used models for studying developmental processes.

    Purpose of the Study:

    • To describe a reliable method for whole mouse embryo visualization using lacZ staining.
    • To enable detailed morphological and molecular analysis of embryonic development.

    Main Methods:

    • Whole mouse embryos were stained for beta-galactosidase (lacZ) activity.
    • Embryos were postfixed in paraformaldehyde.
    • Samples were embedded in agar and visualized using bright- or dark-field microscopy.

    Main Results:

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    Related Experiment Videos

    Last Updated: Jun 4, 2026

    Tracing Gene Expression Through Detection of β-galactosidase Activity in Whole Mouse Embryos
    08:42

    Tracing Gene Expression Through Detection of β-galactosidase Activity in Whole Mouse Embryos

    Published on: June 26, 2018

    Whole-mount Imaging of Mouse Embryo Sensory Axon Projections
    08:37

    Whole-mount Imaging of Mouse Embryo Sensory Axon Projections

    Published on: December 9, 2014

    Contrast Imaging in Mouse Embryos Using High-frequency Ultrasound
    10:39

    Contrast Imaging in Mouse Embryos Using High-frequency Ultrasound

    Published on: March 4, 2015

    • The described protocol allows for clear visualization of lacZ activity in whole mouse embryos.
    • Microscopy revealed detailed spatial patterns of gene expression within the embryonic structures.

    Conclusions:

    • This method provides a robust approach for studying gene expression patterns during mouse embryogenesis.
    • The technique facilitates the integration of molecular and morphological data in developmental studies.