Quantitative microinjection of Drosophila embryos: general strategy

INTRODUCTIONMicroinjection of Drosophila embryos is a common technique used by a wide range of investigators, but some applications require a refined strategy for handling embryos. This article outlines the general procedures for microinjection and quantification of aqueous solutions during high-resolution observation of early development in the fly embryo. It also describes the design of suitable support slides for the manipulation of Drosophila embryos under upright and inverted microscopes.