An approach for immunofluorescence of Drosophila s2 cells

INTRODUCTIONImmunofluorescence is widely used as a technique for visualization of a specific protein in cells or tissue sections. The two major types of immunofluorescence staining methods are (1) direct immunofluorescence staining, in which the primary antibody is labeled with fluorescence dye, and (2) indirect immunofluorescence staining, in which a secondary antibody labeled with fluorochrome is used to recognize a primary antibody. Immunofluorescence staining can be performed on whole tissue, tissue sections, or cells fixed on slides. Despite the availability of various immunofluorescence protocols, it is still a challenge to work with Drosophila S2 cells because of their poor attachment and comparatively low permeability. Here we provide a modified and standardized immunofluorescence protocol specifically for S2 cells.