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Related Concept Videos

FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...

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Related Experiment Video

Updated: Jun 4, 2026

Culture and Transfection of Zebrafish Primary Cells
08:51

Culture and Transfection of Zebrafish Primary Cells

Published on: August 17, 2018

RNA FISH on Cultured Cells in Interphase.

Ng Karen1, Anton Wutz

  • 1Research Institute of Molecular Pathology (IMP), 1030 Vienna, Austria.

CSH Protocols
|March 2, 2011
PubMed
Summary

Fluorescence in situ hybridization (FISH) visualizes RNA localization within cells. This method precisely maps Xist RNA, a key transcript for chromosome regulation, offering insights into gene expression and cellular function.

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Fluorescence in situ hybridization (FISH) is a versatile technique for genome and molecular genetic studies.
  • RNA FISH specifically analyzes intracellular RNA localization, providing insights into transcription and transport within cells.
  • Xist RNA localization is studied using RNA FISH for precise molecular information within cellular structures.

Purpose of the Study:

  • To detail the application of RNA FISH for studying Xist RNA localization.
  • To highlight the advantages of RNA FISH in extracting precise molecular data in cellular contexts.
  • To provide a foundation for understanding RNA localization studies in molecular genetics.

Main Methods:

  • Generation of a labeled probe specific to the target RNA (Xist RNA).

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Robust 3D DNA FISH Using Directly Labeled Probes
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Robust 3D DNA FISH Using Directly Labeled Probes

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Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization
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Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization

Published on: February 3, 2013

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Last Updated: Jun 4, 2026

Culture and Transfection of Zebrafish Primary Cells
08:51

Culture and Transfection of Zebrafish Primary Cells

Published on: August 17, 2018

Robust 3D DNA FISH Using Directly Labeled Probes
12:16

Robust 3D DNA FISH Using Directly Labeled Probes

Published on: August 15, 2013

Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization
13:55

Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization

Published on: February 3, 2013

  • Hybridization of the labeled probe to a fixed cellular sample.
  • Detection of the hybridized probe using microscopy to determine RNA location.

    • Main Results:

    • Successful detection of Xist RNA localization within the cell nucleus.
    • Demonstration of RNA FISH's capability to provide precise spatial information about RNA molecules.
    • Visualization of Xist RNA's association with the chromosome from which it is transcribed.

    Conclusions:

    • RNA FISH is an effective method for studying the localization of specific transcripts like Xist RNA.
    • The technique allows for detailed analysis of RNA's role in cellular processes and gene regulation.
    • Precise localization data obtained via RNA FISH aids in understanding complex genetic mechanisms.