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Related Experiment Video

Updated: Jun 4, 2026

Cell Lineage Analyses and Gene Function Studies Using Twin-spot MARCM

Cell Lineage Analyses and Gene Function Studies Using Twin-spot MARCM

Published on: March 2, 2017

Single-Neuron Labeling Using the Genetic MARCM Method.

|March 2, 2011

Summary

This summary is machine-generated.

This protocol details single-neuron labeling using the MARCM (Mosaic Analysis with a Repressible Cell Marker) system. This method aids in visualizing individual neurons within complex brain structures.

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Labeling of Single Cells in the Central Nervous System of Drosophila melanogaster

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Targeted Labeling of Neurons in a Specific Functional Micro-domain of the Neocortex by Combining Intrinsic Signal and Two-photon Imaging

Targeted Labeling of Neurons in a Specific Functional Micro-domain of the Neocortex by Combining Intrinsic Signal and Two-photon Imaging

Published on: December 12, 2012

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Last Updated: Jun 4, 2026

Cell Lineage Analyses and Gene Function Studies Using Twin-spot MARCM

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Published on: March 2, 2017

Labeling of Single Cells in the Central Nervous System of Drosophila melanogaster

Labeling of Single Cells in the Central Nervous System of Drosophila melanogaster

Published on: March 4, 2013

Targeted Labeling of Neurons in a Specific Functional Micro-domain of the Neocortex by Combining Intrinsic Signal and Two-photon Imaging

Targeted Labeling of Neurons in a Specific Functional Micro-domain of the Neocortex by Combining Intrinsic Signal and Two-photon Imaging

Published on: December 12, 2012

Area of Science:

Neuroscience
Developmental Biology
Genetics

Background:

The brain comprises billions of neurons with intricate structures and connections.
Visualizing individual neurons is crucial for understanding neural complexity.

Purpose of the Study:

To describe a protocol for single-neuron labeling.
To introduce the MARCM (Mosaic Analysis with a Repressible Cell Marker) system for neuronal visualization.

Main Methods:

Utilizes the MARCM system for genetic labeling of single neurons.
Protocol developed initially in Drosophila.

Main Results:

Provides a method for isolating and visualizing individual neurons.
Enables detailed study of neuronal morphology and connectivity.

Conclusions:

The MARCM system offers a valuable tool for single-neuron analysis.
The protocol has potential applicability in various model organisms like C. elegans, zebrafish, and mice.