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Updated: Jun 4, 2026

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High Resolution Fluorescent In Situ Hybridization in Drosophila Embryos and Tissues Using Tyramide Signal Amplification
Published on: October 19, 2017
In situ hybridization to Drosophila testes
1Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.
CSH Protocols
|March 2, 2011
Abstract:
INTRODUCTIONThe tips of Drosophila testes harbor the germ line stem cells, their amplifying daughters, and the cells of the stem cell lineage environments. These cells, located within the apical 100 μm, are specifically hard to label, as the testes appear to be less penetrable at the tips. Here we report an in situ hybridization protocol that works reliably to detect RNA in the cells at the apical tips of Drosophila testes.

