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DNA Isolation

DNA Isolation

DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.

DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

DNA Agarose Gel Electrophoresis

DNA Agarose Gel Electrophoresis

Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...

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Related Experiment Video

Updated: Jun 4, 2026

Identifying DNA Mutations in Purified Hematopoietic Stem/Progenitor Cells

Identifying DNA Mutations in Purified Hematopoietic Stem/Progenitor Cells

Published on: February 24, 2014

Preparing DNA from cell pellets for genotyping.

Amrik Sahota¹, Andrew I Brooks, Jay A Tischfield

¹Rutgers University Cell and DNA Repository, Department of Genetics, Piscataway, NJ 08854-8082, USA.

|March 2, 2011

Summary

This summary is machine-generated.

This protocol details large-scale DNA extraction from Epstein-Barr virus-infected human lymphoblast cell lines. It offers methods using either in-house or commercial reagents for efficient DNA isolation.

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Last Updated: Jun 4, 2026

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Extraction of High Molecular Weight Genomic DNA from Soils and Sediments

Published on: November 10, 2009

Area of Science:

Molecular Biology
Genomics

Background:

Epstein-Barr virus (EBV) is commonly used to establish human lymphoblast cell lines (LCLs).
Large-scale DNA extraction is crucial for various downstream genomic applications.

Purpose of the Study:

To describe a protocol for large-scale DNA extraction from LCLs.
To compare in-house and commercial (PUREGENE) reagent methods.

Main Methods:

Utilizing cell pellets from EBV-established LCLs.
Performing DNA extraction using in-house reagents.
Performing DNA extraction using commercial PUREGENE reagents.

Main Results:

The protocol facilitates efficient DNA isolation from LCLs.
Both in-house and commercial methods yield DNA suitable for further analysis.

Conclusions:

A robust protocol for large-scale DNA extraction from EBV-infected LCLs is presented.
The described methods provide options for researchers based on reagent availability and preference.