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Updated: Jun 4, 2026

Genotyping of Plant and Animal Samples without Prior DNA Purification
Published on: September 24, 2012
Preparing DNA from Mammalian sources for genotyping
Amrik Sahota1, Andrew I Brooks, Jay A Tischfield
1Rutgers University Cell and DNA Repository, Department of Genetics, Piscataway, NJ 08854-8082, USA.
Abstract:
INTRODUCTIONThe availability of high-quality DNA from a large number of individuals is a prerequisite for the success of genetic variation studies. This requirement has spurred major technological advances in DNA extraction methodologies. Twenty years ago, large-scale manual extractions took >3 d to complete. Large-scale preparations can now be completed within 3 h using automated extraction platforms, without the need for toxic reagents and often with higher yields than in the past. This article introduces methods and considerations for the extraction of DNA for genotyping, and for the determination of DNA quantity and quality.
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