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Related Experiment Video

Updated: Jun 4, 2026

An Allele-specific Gene Expression Assay to Test the Functional Basis of Genetic Associations
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Genotyping by Allele-Specific Amplification (KASPar).

Edwin Cuppen1

  • 1Hubrecht Laboratorium, 3584 CT Utrecht, The Netherlands.

CSH Protocols
|March 2, 2011
PubMed
Summary

This study details allele-specific amplification using KASPar chemistry for accurate genotyping. This fluorescence-based method simplifies real-time PCR genotyping without requiring labeled probes.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Genotyping is crucial for genetic analysis and marker-assisted selection.
  • Existing methods may require specialized equipment or labeled probes, increasing complexity and cost.

Purpose of the Study:

  • To describe a protocol for genotyping using allele-specific amplification and fluorescence detection (KASPar).
  • To highlight the compatibility and advantages of the KASPar method for real-time PCR setups.

Main Methods:

  • Utilizes allele-specific amplification (KASPar) for targeted DNA analysis.
  • Employs fluorescence detection for real-time monitoring of amplification.
  • Compatible with standard real-time Polymerase Chain Reaction (PCR) instrumentation.

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Related Experiment Videos

Last Updated: Jun 4, 2026

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10:17

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Published on: November 3, 2010

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry
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Published on: June 21, 2018

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08:25

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Published on: April 8, 2017

Main Results:

  • The KASPar method enables reliable genotyping.
  • The protocol does not require the use of specifically labeled oligonucleotides.
  • Demonstrates compatibility with various real-time PCR platforms.

Conclusions:

  • Allele-specific amplification with KASPar provides a versatile and efficient genotyping solution.
  • The absence of labeled probes simplifies the workflow and reduces costs.
  • This method is suitable for diverse genetic research and diagnostic applications.