Related Experiment Video
Updated: Jun 4, 2026
An Allele-specific Gene Expression Assay to Test the Functional Basis of Genetic Associations
Published on: November 3, 2010
Genotyping by Allele-Specific Amplification (KASPar)
1Hubrecht Laboratorium, 3584 CT Utrecht, The Netherlands.
Abstract:
INTRODUCTIONThis protocol describes the use of allele-specific amplification followed by fluorescence detection (KASPar) for genotyping. The method is compatible with any real-time PCR setup and has the advantage that no specifically labeled oligonucleotides are needed.
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