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Updated: Jun 4, 2026
08:56
In Situ Detection of Ribonucleoprotein Complex Assembly in the C. elegans Germline using Proximity Ligation Assay
Published on: May 5, 2020
Genotyping by Oligonucleotide Ligation Assay (OLA)
1Department of Ecology and Evolutionary Biology, University of Kansas, Lawrence, Kansas 66045, USA.
CSH Protocols
|March 2, 2011
Abstract:
INTRODUCTIONThis protocol describes the oligonucleotide ligation assay (OLA), which uses a set of three oligonucleotides, in combination with a thermostable Taq DNA ligase enzyme, to discriminate single-nucleotide polymorphism (SNP) alleles. Sixteen-plex OLA genotyping reactions are carried out, and allele-specific OLA products are detected on membrane arrays using radiolabeled probes.
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