Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
General Transcription Factors01:30

General Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Minimally invasive pancreatectomy with mesentericoportal venous resection: Results from a French national cohort.

HPB : the official journal of the International Hepato Pancreato Biliary Association·2026
Same author

Decision tree-based prediction of 5-year weight trajectories after bariatric surgery in adolescents and young adults: a retrospective cohort study from France and Sweden.

International journal of obesity (2005)·2026
Same author

Metabolic state determines the brain and direct islet effects of liraglutide on enhanced insulin secretion.

Diabetologia·2026
Same author

Predicting Best Performers After Minimally Invasive Left Pancreatectomy: Insights From a National Cohort.

Annals of surgery·2026
Same author

Surgical management of gastroesophageal reflux disease after sleeve gastrectomy with satisfactory weight loss: a SOFFCO-MM practice survey.

Langenbeck's archives of surgery·2026
Same author

Progressive HNF1A-MODY pathophysiology revealed by a translational mouse model.

JCI insight·2026

Related Experiment Video

Updated: Jun 4, 2026

Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing (ChIP-seq)
09:52

Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing (ChIP-seq)

Published on: April 19, 2013

TCF7L2 splice variants have distinct effects on beta-cell turnover and function.

Olivier Le Bacquer1, Luan Shu, Marion Marchand

  • 1CNRS UMR 8199, Institut de Biologie de Lille, Univ Lille Nord de France, France.

Human Molecular Genetics
|March 2, 2011
PubMed
Summary

Type 2 diabetes involves beta-cell failure. Specific TCF7L2 gene variants, TCF7L2 (T-cell factor 7-like 2), impact beta-cell survival and function, potentially explaining disease mechanisms.

More Related Videos

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
11:48

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts
11:19

Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts

Published on: October 9, 2016

Related Experiment Videos

Last Updated: Jun 4, 2026

Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing (ChIP-seq)
09:52

Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing (ChIP-seq)

Published on: April 19, 2013

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
11:48

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts
11:19

Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts

Published on: October 9, 2016

Area of Science:

  • Endocrinology
  • Molecular Biology
  • Genetics

Background:

  • Type 2 diabetes (T2DM) is characterized by beta-cell dysfunction and apoptosis.
  • Genetic predisposition and environmental factors contribute to T2DM.
  • Polymorphisms in TCF7L2 (T-cell factor 7-like 2) are strongly associated with T2DM risk.

Purpose of the Study:

  • To investigate the mechanisms behind TCF7L2 dysregulation in T2DM.
  • To elucidate the distinct roles of TCF7L2 splice variants in beta-cell function and survival.
  • To understand how TCF7L2 impacts Wnt signaling and beta-cell homeostasis.

Main Methods:

  • Analysis of human isolated islets.
  • Identification and characterization of different TCF7L2 splice variants (e.g., B1, B3, B7).
  • Assessment of beta-cell apoptosis, function, and Wnt signaling pathway activation.

Main Results:

  • TCF7L2 transcripts exhibit opposing effects on beta-cell survival and function.
  • TCF7L2 clone B1 (lacking exons 13-16) induced apoptosis and impaired function.
  • TCF7L2 clones B3 and B7 (containing exon 13) enhanced beta-cell survival and function.
  • TCF7L2 mRNA instability and rapid degradation observed under pro-diabetic conditions.
  • TCF7L2 depletion activated glycogen synthase kinase 3-beta, independent of ER stress.

Conclusions:

  • Function-specific TCF7L2 transcripts have distinct physiological and pathophysiological roles.
  • Deleterious TCF7L2 splice variants may contribute to beta-cell failure in T2DM.
  • Understanding TCF7L2 variants offers insights into T2DM pathogenesis.