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c-Myb in Smooth Muscle Cells

M Simons1, R D Rosenberg

  • 1Department of Biology, Massachusetis Institute of Technology, Cambridge, MA.

Methods in Molecular Medicine
|March 2, 2011
PubMed

Insights

The proto-oncogene c-myb, a homolog of viral oncogene v-myb, is conserved in eukaryotes. Human c-myb mRNA in thymus suggests a potential intermolecular recombination event in its formation.

Area of Science:

  • Molecular Biology
  • Genetics
  • Oncology

Background:

  • The c-myb gene is a proto-oncogene and a cellular homolog of the viral oncogene v-myb.
  • v-myb is found in avian acute leukemia viruses, including avian myeloblastosis virus (AMV) and E26 leukemia virus.
  • The myb gene is highly conserved across eukaryotic species.

Purpose of the Study:

  • To investigate the genomic structure and expression of the c-myb gene.
  • To explore potential mechanisms involved in the formation of mature c-myb protein in humans.

Main Methods:

  • Analysis of gene structure and conservation.
  • Mapping of the human c-myb gene locus.
  • Detection and characterization of c-myb mRNA transcripts in human thymus.

Main Results:

  • The c-myb gene typically comprises 15 exons spanning over 35 kb of genomic DNA.
  • The human c-myb gene locus is mapped to chromosome 6 (6q22-23).
  • c-myb mRNA in human thymus shows transcripts originating from chromosome 17 (17q25).

Conclusions:

  • The findings suggest a potential intermolecular recombinant event in the formation of mature c-myb protein in human thymus.
  • The conservation of the myb gene highlights its fundamental biological importance.
  • Understanding c-myb gene expression is crucial for research in leukemia and cancer biology.

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