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c-Myb in Smooth Muscle Cells
1Department of Biology, Massachusetis Institute of Technology, Cambridge, MA.
Methods in Molecular Medicine
|March 2, 2011
Summary
The proto-oncogene c-myb, a homolog of viral oncogene v-myb, is conserved in eukaryotes. Human c-myb mRNA in thymus suggests a potential intermolecular recombination event in its formation.
Area of Science:
- Molecular Biology
- Genetics
- Oncology
Background:
- The c-myb gene is a proto-oncogene and a cellular homolog of the viral oncogene v-myb.
- v-myb is found in avian acute leukemia viruses, including avian myeloblastosis virus (AMV) and E26 leukemia virus.
- The myb gene is highly conserved across eukaryotic species.
Purpose of the Study:
- To investigate the genomic structure and expression of the c-myb gene.
- To explore potential mechanisms involved in the formation of mature c-myb protein in humans.
Main Methods:
- Analysis of gene structure and conservation.
- Mapping of the human c-myb gene locus.
- Detection and characterization of c-myb mRNA transcripts in human thymus.
Main Results:
- The c-myb gene typically comprises 15 exons spanning over 35 kb of genomic DNA.
- The human c-myb gene locus is mapped to chromosome 6 (6q22-23).
- c-myb mRNA in human thymus shows transcripts originating from chromosome 17 (17q25).
Conclusions:
- The findings suggest a potential intermolecular recombinant event in the formation of mature c-myb protein in human thymus.
- The conservation of the myb gene highlights its fundamental biological importance.
- Understanding c-myb gene expression is crucial for research in leukemia and cancer biology.
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