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Related Experiment Video

Updated: Jun 4, 2026

Lineage Labeling of Zebrafish Cells with Laser Uncagable Fluorescein Dextran
07:35

Lineage Labeling of Zebrafish Cells with Laser Uncagable Fluorescein Dextran

Published on: April 28, 2011

Labelling cell structures and tracking cell lineage in zebrafish using SNAP-tag.

Cláudia Campos1, Mako Kamiya, Sambashiva Banala

  • 1Departement de Biochimie, Université de Genève, Genève, Switzerland.

Developmental Dynamics : an Official Publication of the American Association of Anatomists
|March 2, 2011
PubMed
Summary

This study introduces a novel method for specific labeling of fusion proteins in Zebrafish using SNAP-tag technology and synthetic fluorophores. This technique enables detailed visualization of subcellular structures and facilitates cell lineage tracing.

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Area of Science:

  • Biochemistry
  • Cell Biology
  • Zebrafish Model Organisms

Background:

  • Specific labeling of fusion proteins is crucial for understanding cellular processes.
  • Existing methods often have limitations in stability and compatibility with fixation techniques.

Purpose of the Study:

  • To develop and demonstrate a method for specific labeling of fusion proteins in Zebrafish using synthetic fluorophores.
  • To showcase the utility of this method for visualizing subcellular structures and for cell lineage tracing.

Main Methods:

  • Utilized SNAP-tag technology for protein labeling.
  • Employed O(6)-benzylguanine derivatives of synthetic fluorophores.
  • Demonstrated labeling of nucleus, cell membranes, and endosomal membranes in Zebrafish.

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Labeling and Imaging Cells in the Zebrafish Hindbrain
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Labeling and Imaging Cells in the Zebrafish Hindbrain

Published on: July 25, 2010

Live Imaging of Cell Motility and Actin Cytoskeleton of Individual Neurons and Neural Crest Cells in Zebrafish Embryos
10:52

Live Imaging of Cell Motility and Actin Cytoskeleton of Individual Neurons and Neural Crest Cells in Zebrafish Embryos

Published on: February 3, 2010

Related Experiment Videos

Last Updated: Jun 4, 2026

Lineage Labeling of Zebrafish Cells with Laser Uncagable Fluorescein Dextran
07:35

Lineage Labeling of Zebrafish Cells with Laser Uncagable Fluorescein Dextran

Published on: April 28, 2011

Labeling and Imaging Cells in the Zebrafish Hindbrain
09:17

Labeling and Imaging Cells in the Zebrafish Hindbrain

Published on: July 25, 2010

Live Imaging of Cell Motility and Actin Cytoskeleton of Individual Neurons and Neural Crest Cells in Zebrafish Embryos
10:52

Live Imaging of Cell Motility and Actin Cytoskeleton of Individual Neurons and Neural Crest Cells in Zebrafish Embryos

Published on: February 3, 2010

Main Results:

  • Achieved specific labeling of fusion proteins in Zebrafish.
  • Showcased stable labeling suitable for long-term imaging.
  • Demonstrated compatibility with acid fixatives, unlike autofluorescent proteins.
  • Enabled cell lineage tracing using photo-deprotectable caged fluorescein.

Conclusions:

  • The SNAP-tag and synthetic fluorophore method provides a robust tool for Zebrafish research.
  • This technique enhances imaging capabilities and offers new possibilities for lineage analysis.
  • The method's stability and compatibility expand its applicability in biological studies.