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Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

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Published on: July 25, 2022

The Next Frontier in Vibrational Imaging: Chemically Designed Raman Probes Pass the Spectral Barrier.

Hiroyoshi Fujioka¹, Mako Kamiya¹

¹Laboratory for Chemistry and Life Science, Institute of Integrated Research, Institute of Science Tokyo, Kanagawa 226-8501, Japan.

|May 29, 2026

Summary

Chemically engineered probes enable multicolor vibrational imaging in biological samples. This review categorizes Raman imaging probe designs and discusses their potential for studying complex biological phenomena.

Keywords:

Raman probe alkyne azo deuterium diarylethene nanoparticles polyyne pyronin

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Area of Science:

Biomedical Imaging
Chemical Biology
Spectroscopy

Background:

Multicolor vibrational imaging offers high specificity for analyzing biological systems.
Advancements in microscopy and chemical probe design are driving progress in this field.
Raman imaging provides label-free molecular contrast.

Purpose of the Study:

To review design strategies for Raman imaging probes.
To categorize probes based on their chemical structure.
To discuss the future potential and research needs for multicolor vibrational probes.

Main Methods:

Literature review of recent advancements in Raman imaging probes.
Categorization of probes based on chemical structure and design principles.
Analysis of probe performance in cellular and tissue imaging.

Main Results:

Raman imaging probes can be designed using various chemical strategies.
Different probe designs offer distinct advantages for specific biological applications.
Integration of probes with advanced microscopy enhances imaging capabilities.

Conclusions:

Multicolor vibrational imaging holds significant promise for understanding complex biological processes.
Further research is needed to optimize probe design and expand applications.
Standardized design principles will accelerate the development of novel imaging probes.