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Related Concept Videos

Raman Spectroscopy Instrumentation: Overview01:26

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A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
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Raman Spectroscopy: Overview01:20

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The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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Functional Raman Probes for Detecting Enzyme Activities Based on Aggregation Control.

Momoko Okinaka1, Minoru Kawatani2,3, Hiroyoshi Fujioka2,3

  • 1Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo 113-0033, Japan.

Analytical Chemistry
|August 27, 2025
PubMed
Summary
This summary is machine-generated.

Researchers developed novel Raman imaging probes for visualizing enzyme activity in living systems. These probes aggregate upon enzyme hydrolysis, enhancing Raman signals for clearer detection in cells and spheroids.

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Area of Science:

  • Biomedical research
  • Chemical biology
  • Molecular imaging

Background:

  • Visualizing enzyme activity in living systems is crucial for biomedical research.
  • Raman imaging probes offer multiplexed detection capabilities due to narrow signal peaks.

Purpose of the Study:

  • To present a molecular design strategy for enzyme-activity-detecting Raman probes based on aggregation control.
  • To enable visualization of specific enzyme activities in real-time within biological systems.

Main Methods:

  • Designed Raman probes with hydrophilic substrates that become hydrophobic upon enzyme hydrolysis.
  • Utilized aggregation of the hydrophobic product to amplify Raman signals.
  • Employed isotope-editing for vibrational frequency control.
  • Developed probes targeting aminopeptidase, glycosidase, and carboxypeptidase.

Main Results:

  • The molecular design strategy successfully yielded functional Raman probes.
  • Developed probes demonstrated successful visualization of aminopeptidase and glycosidase activities.
  • Applications were validated in live cultured cells and spheroids.

Conclusions:

  • The aggregation-based molecular design is effective for creating enzyme-activity-detecting Raman probes.
  • These probes offer a powerful tool for visualizing enzyme activities in complex biological environments.
  • The strategy holds potential for advancing multiplexed enzyme activity detection in biomedical research.