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Candida albicans identification: comparison among nine phenotypic systems and a multiplex PCR
G Liguori1, V Di Onofrio, F Gallé
1Department of Studies of Institutions and Territorial Systems, University of Naples Parthenope, Italy. liguori@uniparthenope.it
Journal of Preventive Medicine and Hygiene
|March 3, 2011
Summary
This study evaluated nine phenotypic methods for identifying Candida albicans, a common fungal pathogen. Multiplex PCR confirmed results, showing most methods had acceptable sensitivity and specificity for clinical use.
Area of Science:
- Medical Mycology
- Clinical Microbiology
Background:
- Candida albicans is a prevalent fungal pathogen and commensal, causing significant clinical infections.
- Non-albicans Candida species also contribute to a notable number of infections.
Purpose of the Study:
- To compare the performance of nine commercial phenotypic identification systems against multiplex PCR for Candida albicans detection.
- To assess the sensitivity and specificity of each phenotypic system.
Main Methods:
- A total of 390 yeast isolates from oral and vaginal swabs were analyzed.
- Isolates were subjected to germ tube testing, multiplex PCR, and nine different phenotypic identification methods.
- Comparison focused on the accurate identification of Candida albicans.
Main Results:
- Multiplex PCR confirmed the identity of 253 isolates initially identified as Candida albicans by germ tube test.
- Phenotypic system sensitivity ranged from 81.9% (Vitek2) to 87.7% (Candida ID2 and CHROMagar).
- Specificity varied, with Candida ID2 achieving 96.8% and Chromalbicans Agar 75.1%.
Conclusions:
- All evaluated phenotypic methods demonstrated acceptable sensitivity and specificity compared to multiplex PCR.
- These methods can be valuable for Candida albicans identification in settings lacking molecular diagnostic capabilities.
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