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Raman Spectroscopy Instrumentation: Overview

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A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
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Updated: Jun 4, 2026

Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering

Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering

Published on: July 6, 2019

Improving sensitivity in nonlinear Raman microspectroscopy imaging and sensing.

Rajan Arora¹, Georgi I Petrov, Jian Liu

¹University of Wisconsin - Milwaukee, Department of Physics, 1900 E. Kenwood Boulevard, Milwaukee, Wisconsin 53211, USA.

Journal of Biomedical Optics

|March 3, 2011

Summary

This summary is machine-generated.

Broadband coherent anti-Stokes Raman scattering (CARS) enables noninvasive chemical analysis of cells and tissues. Optimizing laser power significantly enhances imaging sensitivity and allows for high-quality vibrational spectra acquisition.

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A Multimodal Wide-Field Fourier-Transform Raman Microscope

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

Area of Science:

Biomedical Optics
Chemical Imaging
Spectroscopy

Background:

Nonlinear Raman microspectroscopy, particularly broadband coherent anti-Stokes Raman scattering (CARS), is a developing technology.
This technique offers noninvasive, chemically specific microscopic analysis for biological samples like tissues and cells.

Purpose of the Study:

To investigate the critical factors influencing the sensitivity of CARS imaging.
To demonstrate the feasibility of high-quality vibrational spectra acquisition for biomedical applications.

Main Methods:

Utilized broadband coherent anti-Stokes Raman scattering (CARS) microspectroscopy.
Investigated the impact of incident laser power on imaging sensitivity.
Optimized laser system parameters for spectral acquisition.

Main Results:

Incident laser power was identified as the primary parameter controlling CARS imaging sensitivity.
High-quality vibrational spectra acquisition at multi-kHz rates was achieved through laser system optimization.
Demonstrated the potential for sensitive, chemically specific microscopic analysis.

Conclusions:

Optimizing laser power is crucial for enhancing the sensitivity of CARS-based biomedical imaging.
The developed technique enables rapid, high-quality vibrational spectroscopy for analyzing biological samples.
This advancement supports the application of CARS in noninvasive cellular and tissue analysis.