Related Experiment Video
Updated: Jun 4, 2026

Microhardness Measurements on Tooth and Alveolar Bone in Rodent Oral Disease Models
Published on: April 26, 2024
Alkali-soluble fluoride deposition on enamel after professional application of topical fluoride in vitro
Mitsue Fujimaki Hayacibara1, Adriana Franco Paes Leme, Ynara Bosco de Oliveira Lima
1Department of Physiological Sciences, Piracicaba Dental School, Universidade Estadual de Campinas, Brazil.
Abstract:
Since the efficacy of topical fluoride products is related to the fluoride (F) availability and its reactivity with enamel, this study was conducted. The F concentration of the following materials was verified: I- acidulated phosphate fluoride (APF) gel (1.23% F), II- APF foam (1.23% F) and III- Varnish (2.26% F). Forty blocks of bovine enamel were divided into 4 groups and treated according to the materials described, being one of them used as control. Loosely bound fluoride (''CaF2'') was determined on enamel after extraction with 1.0M KOH and analyzed by ion-selective electrode. Total F concentration found in gel was 12,642, in foam 12,755 and in varnish 23,183 mg F/g. All products formed statistically higher amounts of ''CaF2'' on enamel compared to the control group (p < 0.05), but the difference between them was not significant (p > 0.05). Thus, ''CaF2'' formation was not proportional to the total F content in the products, suggesting that the pH and the vehicle used are more important.
