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Bioactive glass 13-93 as a subchondral substrate for tissue-engineered osteochondral constructs: a pilot study
Prakash Jayabalan1, Andrea R Tan, Mohammed N Rahaman
1Comparative Orthopaedic Laboratory, University of Missouri, 900 East Campus Drive, Columbia, MO 65211, USA.
Clinical Orthopaedics and Related Research
|March 3, 2011
Summary
Bioactive Glass 13-93 (BG 13-93) as a culture supplement enhanced tissue-engineered cartilage. However, BG 13-93 did not improve constructs when used as a subchondral substrate for osteoarthritis treatment.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Regenerative Medicine
Background:
- Osteoarthritis treatment involves replacing diseased joint areas with tissue-engineered osteochondral grafts.
- Bioactive glasses are being investigated as potential bone components in these grafts.
Purpose of the Study:
- To evaluate Bioactive Glass 13-93 (BG 13-93) as a subchondral substrate for cartilage layer production.
- To assess BG 13-93 as a culture medium supplement for enhancing cartilage layer collagen and glycosaminoglycan production and mechanical properties.
Main Methods:
- Study 1: Osteochondral constructs with BG 13-93 as a subchondral base were cultured for 6 weeks.
- Study 2: Cell-seeded agarose layers were exposed to BG 13-93 in culture medium temporally (continuous, first 2 weeks, last 2 weeks) or not at all for 6 weeks.
Main Results:
- Constructs with a BG 13-93 base showed increased glycosaminoglycan but decreased collagen II deposition.
- Temporal exposure to BG 13-93 in culture medium improved mechanical and biochemical properties compared to continuous or no exposure.
Conclusions:
- BG 13-93 is not ideal as a subchondral substrate for osteochondral constructs.
- BG 13-93 shows promise as a culture medium supplement to enhance neocartilage formation.
