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¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

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Related Experiment Video

Updated: Jun 4, 2026

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging

Hyperpolarized ¹³C Metabolic Magnetic Resonance Spectroscopy and Imaging

Published on: December 30, 2016

A hydrogen peroxide-responsive hyperpolarized 13C MRI contrast agent.

Alexander R Lippert¹, Kayvan R Keshari, John Kurhanewicz

¹Department of Chemistry, University of California, Berkeley, California 94720, United States.

Journal of the American Chemical Society

|March 4, 2011

Summary

We developed a new method to detect hydrogen peroxide (H2O2) using hyperpolarized carbon-13 (13C) MRI. This technique uses a responsive agent to visualize H2O2 levels non-invasively in preclinical studies.

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Published on: September 13, 2019

Area of Science:

Biomedical Imaging
Chemical Biology
Medical Diagnostics

Background:

Hydrogen peroxide (H2O2) is a key reactive oxygen species involved in various biological processes.
Accurate detection and imaging of H2O2 are crucial for understanding its role in health and disease.
Current methods for H2O2 detection have limitations in sensitivity, specificity, or non-invasive applicability.

Purpose of the Study:

To develop a novel reaction-based approach for detecting hydrogen peroxide (H2O2).
To utilize hyperpolarized carbon-13 (13C) magnetic resonance imaging (MRI) for H2O2 visualization.
To establish a non-invasive imaging methodology for monitoring H2O2 in biological systems.

Main Methods:

Employing a reaction-based strategy involving the H2O2-mediated oxidation of α-ketoacids to carboxylic acids.
Utilizing (13)C-Benzoylformic acid as a selective probe that reacts with H2O2 to form (13)C-benzoic acid.
Leveraging dynamic nuclear polarization (DNP) to hyperpolarize the (13)C-labeled precursor for enhanced MRI signal.
Implementing frequency-specific imaging sequences for dual-frequency detection of H2O2.

Main Results:

Demonstrated selective reaction of (13)C-Benzoylformic acid with H2O2 over other reactive oxygen species.
Successfully generated hyperpolarized (13)C-benzoic acid, enabling sensitive detection.
Acquired phantom images showcasing the responsive contrast agent's efficacy in monitoring H2O2.
Validated the method's performance at pre-clinical magnetic field strengths.

Conclusions:

The developed reaction-based approach combined with hyperpolarized (13)C MRI offers a powerful new tool for H2O2 detection.
This methodology enables non-invasive monitoring of H2O2 levels in biological systems.
The findings pave the way for advanced multi-analyte imaging in living organisms.