Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

State-dependent energy conversion produces degenerate dissipation in active actomyosin networks.

bioRxiv : the preprint server for biology·2026
Same author

Mechanical movements generated by movable lipids break endosomal barriers for enhanced mRNA therapeutics.

Science advances·2026
Same author

A novel translation initiation codon single nucleotide variant of the SLC14A1 (c.2T>G) from a Chinese blood donor with Jk(a-b-) phenotype.

Transfusion·2026
Same author

A pico-calorimeter for cellular metabolism and antimicrobial susceptibility testing.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

High-fidelity compressed high-speed imaging for resolving rapid micro-dynamics.

Optics express·2026
Same author

Inverse-designed silicon nitride nanophotonics.

Nature communications·2026

Related Experiment Video

Updated: Jun 4, 2026

High-speed Continuous-wave Stimulated Brillouin Scattering Spectrometer for Material Analysis
07:55

High-speed Continuous-wave Stimulated Brillouin Scattering Spectrometer for Material Analysis

Published on: September 22, 2017

Parallel two-step phase-shifting point-diffraction interferometry for microscopy based on a pair of cube

Peng Gao1, Baoli Yao, Junwei Min

  • 1State Key Laboratory of Transient Optics and Photonics, Xi'an Institute of Optics and Precision Mechanics,Chinese Academy of Sciences, Xi'an 710119, China.

Optics Express
|March 4, 2011
PubMed
Summary
This summary is machine-generated.

A novel parallel phase-shifting interferometry technique for microscopy uses cube beamsplitters to create two parallel interferograms. This method enhances stability and is ideal for imaging dynamic processes and moving objects.

More Related Videos

A Multimodal Wide-Field Fourier-Transform Raman Microscope
06:48

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

Measurement of X-ray Beam Coherence along Multiple Directions Using 2-D Checkerboard Phase Grating
10:39

Measurement of X-ray Beam Coherence along Multiple Directions Using 2-D Checkerboard Phase Grating

Published on: October 11, 2016

Related Experiment Videos

Last Updated: Jun 4, 2026

High-speed Continuous-wave Stimulated Brillouin Scattering Spectrometer for Material Analysis
07:55

High-speed Continuous-wave Stimulated Brillouin Scattering Spectrometer for Material Analysis

Published on: September 22, 2017

A Multimodal Wide-Field Fourier-Transform Raman Microscope
06:48

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

Measurement of X-ray Beam Coherence along Multiple Directions Using 2-D Checkerboard Phase Grating
10:39

Measurement of X-ray Beam Coherence along Multiple Directions Using 2-D Checkerboard Phase Grating

Published on: October 11, 2016

Area of Science:

  • Optics and Photonics
  • Microscopy Techniques
  • Interferometry

Background:

  • Phase-shifting interferometry is crucial for high-resolution imaging.
  • Traditional methods can be sensitive to environmental vibrations, limiting applications.
  • Common-path configurations offer enhanced stability for interferometric setups.

Purpose of the Study:

  • To propose a novel parallel two-step phase-shifting point-diffraction interferometry system.
  • To enhance stability and applicability for dynamic process measurements.
  • To suppress the DC term for improved image quality.

Main Methods:

  • Utilizing a pair of cube beamsplitters for wave splitting and recombination.
  • Implementing a pinhole filter in the Fourier plane to generate a reference wave.
  • Employing polarization elements to obtain two parallel phase-shifting interferograms.
  • Performing slightly-off-axis interferometry by subtracting the generated holograms.

Main Results:

  • Achieved a stable, common-path interferometric setup.
  • Successfully suppressed the DC term through hologram subtraction.
  • Demonstrated suitability for measuring moving objects and dynamic processes.
  • Obtained two parallel phase-shifting interferograms.

Conclusions:

  • The proposed parallel phase-shifting point-diffraction interferometry offers a robust and stable microscopy solution.
  • The common-path design and DC term suppression are key advantages for dynamic measurements.
  • This technique advances the capability of interferometric microscopy for real-time imaging applications.