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Related Concept Videos

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
Telomeres and Telomerase02:41

Telomeres and Telomerase

In eukaryotic DNA replication, a single-stranded DNA fragment remains at the end of a chromosome after the removal of the final primer. This section of DNA cannot be replicated in the same manner as the rest of the strand because there is no 3’ end to which the newly synthesized DNA can attach. This non-replicated fragment results in gradual loss of the chromosomal DNA during each cell duplication. Additionally, it can induce a DNA damage response by enzymes that recognize single-stranded DNA.
PCR - Polymerase Chain Reaction01:32

PCR - Polymerase Chain Reaction

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Related Experiment Video

Updated: Jun 4, 2026

Monochrome Multiplex Quantitative PCR Telomere Length Measurement
11:44

Monochrome Multiplex Quantitative PCR Telomere Length Measurement

Published on: March 22, 2024

A quantitative PCR method for measuring absolute telomere length.

Nathan J O'Callaghan1, Michael Fenech

  • 1CSIRO Food and Nutritional Sciences, Nutritional Genomics Laboratory, PO Box 10041, Adelaide BC, SA, 5000, Australia.

Biological Procedures Online
|March 4, 2011
PubMed
Summary
This summary is machine-generated.

We present a novel quantitative real-time PCR method for measuring absolute telomere length (aTL). This reproducible assay uses an oligomer standard for accurate aTL quantification, improving inter-laboratory comparisons.

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Optimization of Performance Parameters of the TAGGG Telomere Length Assay
08:23

Optimization of Performance Parameters of the TAGGG Telomere Length Assay

Published on: April 21, 2023

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Telomere length (TL) is a crucial biomarker in aging and disease.
  • Existing methods for TL measurement, particularly relative quantitative PCR (qPCR), lack standardization.
  • Absolute telomere length (aTL) offers more precise and comparable data.

Purpose of the Study:

  • To develop and validate a simple, reproducible method for absolute telomere length (aTL) measurement using qPCR.
  • To provide a standardized protocol for aTL quantification applicable to molecular epidemiology.
  • To enable direct comparison of TL data across different experiments and laboratories.

Main Methods:

  • Adaptation of the Cawthon method for relative TL measurement.
  • Introduction of an oligomer standard for absolute quantification.
  • Detailed description of standard curve generation and data calculation from qPCR.

Main Results:

  • The developed method allows for high-throughput aTL measurement using minimal DNA amounts.
  • The assay demonstrates robust performance characteristics, detailed with controls.
  • Results are compared to existing TL measurement techniques, highlighting improved comparability.

Conclusions:

  • This protocol provides a reliable method for absolute telomere length (aTL) determination.
  • The standardization offered by this aTL qPCR assay facilitates large-scale molecular epidemiological studies.
  • The method enhances the comparability and reproducibility of telomere length measurements globally.