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Published on: September 29, 2021
Histopathologic response to highly purified liquid silicone injected intradermally in rats' skin
Ozcan Cakmak1, Huseyin Kemal Turkoz, Senol Polat
1Department of Otorhinolaryngology, Acibadem University School of Medicine, Kadikoy, 34718 Istanbul, Turkey. ozcan.cakmak@gmail.com
Background:
This study aimed to assess and compare the tissue response to small- and large-volume injections of pure liquid silicone in rats.
Methods:
The study used 25 Sprague-Dawley rats. Two different injection sites were determined for a single rat, and 5000 centistoke liquid silicone was injected subdermally using 0.2-0.3 ml (small volume) and 1 ml (large volume) injections. After the injections, 15 animals were killed at 4 weeks and 10 animals at 10 weeks. Tissue responses were evaluated under a light microscope.
Results:
At week 4, the numbers of lymphocytes and neutrophils were higher in the small-volume injection group. At week 10, the number of neutrophils decreased, and the numbers of macrophages and foreign body giant cells increased in the small-volume injection group, whereas the intensity and type of infiltrate in the large-volume injection group did not change significantly. The thickness of the fibrous capsule was greater in large-volume injection group at weeks 4 and 10. The thickness of the fibrous capsule did not change significantly in either group through time.
Conclusions:
Purified high-density liquid injectable silicone causes a low-grade and well-tolerated inflammatory response during the long term and can be used when given as small-volume injections.
