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Updated: Jun 4, 2026

High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture (4C-seq)

High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture (4C-seq)

Published on: October 5, 2018

Computational analysis of high throughput sequencing data.

Steve Hoffmann¹

¹Interdisciplinary Center for Bioinformatics and The Junior Research Group for Transcriptome Bioinformatics in the LIFE Research Cluster, University Leipzig, Leipzig, Germany. steve@bioinf.uni-leipzig.de

Methods in Molecular Biology (Clifton, N.J.)

|March 4, 2011

Summary

This summary is machine-generated.

High Throughput Sequencing (HTS) rapidly analyzes genomes and transcriptomes, enabling personalized genomic medicine. HTS offers new insights into RNA, structural variants, and splicing.

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High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture (4C-seq)

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Area of Science:

Genomics
Bioinformatics
Molecular Biology

Background:

High Throughput Sequencing (HTS) has revolutionized biological data analysis.
Mammalian genome sequencing has dramatically decreased in time and cost.
Personalized genomic medicine is a key driver for HTS advancements.

Purpose of the Study:

To provide an overview of High Throughput Sequencing technologies.
To discuss the bioinformatics analysis of HTS data.
To highlight the impact of HTS on genomic and transcriptomic research.

Main Methods:

Review of current High Throughput Sequencing platforms.
Discussion of computational approaches for analyzing large-scale sequencing data.
Exploration of applications in noncoding RNA discovery, structural variant detection, and alternative splicing analysis.

Main Results:

HTS enables rapid genome and transcriptome analysis, significantly reducing sequencing time.
New insights are gained into noncoding RNAs, structural variants, and alternative splicing.
Various HTS technologies are now commercially available.

Conclusions:

High Throughput Sequencing offers unprecedented opportunities in genomic and transcriptomic research.
The advancement of HTS technologies accelerates personalized genomic medicine.
Bioinformatics analysis is crucial for interpreting complex HTS data.