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Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

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Related Experiment Video

Updated: Jun 3, 2026

Isolation and Quantification of Axonal mRNAs Using Porous Membrane Inserts and RTddPCR

Isolation and Quantification of Axonal mRNAs Using Porous Membrane Inserts and RTddPCR

Published on: February 6, 2026

Measuring MDR-1 by Quantitative RT-PCR.

S E Bates¹, Z Zhan, J Regis

¹Medicine Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD.

Methods in Molecular Medicine

|March 5, 2011

Summary

This summary is machine-generated.

Measuring P-glycoprotein and MDR-1 mRNA is crucial for understanding drug resistance. This knowledge aids in developing P-glycoprotein antagonists to overcome resistance in clinical settings.

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Last Updated: Jun 3, 2026

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Area of Science:

Biochemistry
Molecular Biology
Clinical Pharmacology

Background:

P-glycoprotein and its encoding MDR-1 mRNA are key indicators of multidrug resistance.
Understanding P-glycoprotein's role is vital for developing effective cancer therapies.

Purpose of the Study:

To emphasize the importance of accurately measuring P-glycoprotein and MDR-1 mRNA expression.
To highlight the role of P-glycoprotein in clinical drug resistance.
To guide the development of P-glycoprotein antagonists.

Main Methods:

Measuring MDR-1 mRNA expression.
Quantifying P-glycoprotein levels.
Performing reversal studies in relevant disease models.

Main Results:

Accurate determination of P-glycoprotein and MDR-1 mRNA expression is essential for assessing drug resistance mechanisms.
Identifying diseases where P-glycoprotein is the major resistance factor is critical for targeted therapies.

Conclusions:

Measuring MDR-1 mRNA or P-glycoprotein in tumors at diagnosis, recurrence, and during treatment is the clearest approach.
This approach will facilitate the development of P-glycoprotein antagonists to overcome drug resistance.