Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Nephrons01:10

Nephrons

The kidneys are intricate organs with millions of working units known as nephrons. Each nephron features two major structures: the renal corpuscle, which facilitates blood plasma filtration, and the renal tubule, which handles the glomerular filtrate. Blood supply is directly linked to the nephrons. The renal corpuscle consists of the glomerulus, a capillary network, and the Bowman's capsule, a double-walled epithelial structure that encases the glomerulus. The filtering of blood plasma happens...
Kidney Structure01:45

Kidney Structure

The kidneys are two large bean-shaped organs located in the upper abdomen. They filter the blood several times a day to remove toxins and rebalance water and electrolytes of the circulatory system via the renal veins. The kidneys receive blood directly from the heart via the renal arteries. These arteries enter the kidney at the hilum, the concave surface of the bean, where they branch and divide into smaller vessels and capillaries.
Internal Anatomy of the Kidney01:12

Internal Anatomy of the Kidney

The kidneys are essential organs in the human body, performing a myriad of tasks that maintain homeostasis and overall health.
Anatomical Position and Dimensions
The kidneys are retroperitoneal organs positioned against the posterior abdominal wall on either side of the spine, roughly between the twelfth thoracic and third lumbar vertebrae. Each kidney is typically 10-12 cm long, 5-6 cm wide, and 3-4 cm thick, weighing about 150 grams.
Renal Cortex
The outermost region of the kidney is the...
Renal Corpuscle01:20

Renal Corpuscle

The glomerulus and Bowman's capsule are two essential components of the nephron, which is the functional unit of the kidney. These microscopic structures play a critical role in the process of blood filtration to produce urine.
Glomerulus: Structure and Function
The glomerulus is a tiny, intricate network of capillaries located at the beginning of the nephron. It's enveloped by the Bowman's capsule and receives its blood supply from an afferent arteriole, which divides into numerous capillaries...
Renal Tubule and Collecting Duct01:24

Renal Tubule and Collecting Duct

The renal tubule is divided into three parts: the proximal convoluted tubule (PCT), the Loop of Henle (LOH), and the distal convoluted tubule (DCT).
Proximal Convoluted Tubule (PCT):
The PCT is the initial segment of the renal tubule, extending from the Bowman's capsule that encloses the glomerulus. Its convoluted structure and microvilli-lined cells increase the surface area for reabsorption. The PCT reabsorbs glucose, amino acids, sodium, and water from the filtrate, ensuring essential...
Renal Drug Excretion: Overview01:15

Renal Drug Excretion: Overview

As primary excretory organs, the kidneys maintain homeostasis by removing waste substances from the bloodstream. They comprise over a million units called nephrons, which serve as the kidney's functional units.
A nephron consists of two primary structures: the renal corpuscle and the renal tubule. The renal corpuscle contains the glomerulus, a network of capillaries where the first step of renal excretion, glomerular filtration, occurs. Blood pressure forces water, ions, and small molecules out...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The neonatal preventable harm index: a high reliability tool.

Journal of perinatology : official journal of the California Perinatal Association·2016
Same author

The use of defined media in cell and tissue culture.

Toxicology in vitro : an international journal published in association with BIBRA·2010
Same author

Application of enzymatically stable dipeptides for enhancement of intestinal permeability. Synthesis and in vitro evaluation of dipeptide-coupled compounds.

Bioorganic & medicinal chemistry·2001
Same author

Realism of confidence in obsessive-compulsive checkers.

Journal of abnormal psychology·2001
Same author

Regulation of phosphate uptake in primary cultured rabbit renal proximal tubule cells by glucocorticoids: evidence for nongenomic as well as genomic mechanisms.

Endocrinology·2001
Same author

Mechanism of regulation of Na+ transport by angiotensin II in primary renal cells.

Kidney international·2000
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Jun 3, 2026

A Novel Clinical Grade Isolation Method for Human Kidney Perivascular Stromal Cells
09:05

A Novel Clinical Grade Isolation Method for Human Kidney Perivascular Stromal Cells

Published on: August 7, 2017

Primary kidney cells.

M Taub1

  • 1State University of New York at Buffalo, Buffalo, New York.

Methods in Molecular Biology (Clifton, N.J.)
|March 5, 2011
PubMed
Summary
This summary is machine-generated.

Serum-free media support kidney epithelial cell cultures, including primary cells. This advancement allows for more accurate in vitro studies that better correlate with animal models.

More Related Videos

Guided Differentiation of Mature Kidney Podocytes from Human Induced Pluripotent Stem Cells Under Chemically Defined Conditions
08:06

Guided Differentiation of Mature Kidney Podocytes from Human Induced Pluripotent Stem Cells Under Chemically Defined Conditions

Published on: July 2, 2020

Related Experiment Videos

Last Updated: Jun 3, 2026

A Novel Clinical Grade Isolation Method for Human Kidney Perivascular Stromal Cells
09:05

A Novel Clinical Grade Isolation Method for Human Kidney Perivascular Stromal Cells

Published on: August 7, 2017

Guided Differentiation of Mature Kidney Podocytes from Human Induced Pluripotent Stem Cells Under Chemically Defined Conditions
08:06

Guided Differentiation of Mature Kidney Podocytes from Human Induced Pluripotent Stem Cells Under Chemically Defined Conditions

Published on: July 2, 2020

Area of Science:

  • Cell Biology
  • Renal Physiology
  • Biotechnology

Background:

  • Established kidney epithelial cell lines (MDCK, LLC-PK1) can be cultured in serum-free conditions.
  • Serum-free media offer advantages for kidney cell research.

Purpose of the Study:

  • To detail the development of hormonally defined serum-free media for kidney epithelial cell cultures.
  • To highlight the benefits of using serum-free conditions for both established cell lines and primary kidney cell cultures.

Main Methods:

  • Culturing established kidney epithelial cell lines (MDCK, LLC-PK1) in serum-free media.
  • Developing serum-free media protocols for primary kidney epithelial cell cultures.

Main Results:

  • Demonstrated successful growth of established kidney cell lines in serum-free environments.
  • Showcased the ability to grow primary kidney epithelial cells in serum-free media.

Conclusions:

  • Hormonally defined serum-free media are effective for kidney epithelial cell growth and functional studies.
  • Serum-free primary kidney cell cultures offer improved correlation with in vivo animal studies and allow for development of more representative in vitro models.