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Updated: Jun 3, 2026
08:48
High-throughput Physical Mapping of Chromosomes using Automated in situ Hybridization
Published on: June 28, 2012
Gene mapping to chromosomes by hybridization in situ
1MRC Clinical and Population Cytogenics Unit, Western General Hospital, Edinburgh, Scotland.
Methods in Molecular Biology (Clifton, N.J.)
|March 5, 2011
Summary
Hybridization in situ, a technique refined since 1969, initially detected only highly repeated DNA sequences like satellite DNA and ribosomal DNA genes.
Area of Science:
- Molecular Biology
- Genetics
- Cytology
Background:
- Hybridization in situ (HIS) was first described in 1969.
- Early HIS techniques were limited to detecting highly repeated DNA sequences.
Purpose of the Study:
- To provide historical context on the evolution of hybridization in situ techniques.
- To highlight the initial limitations and scope of early HIS applications.
Main Methods:
- Historical review of scientific literature.
- Description of early hybridization in situ methodologies.
Main Results:
- Early HIS could only detect and locate highly repeated target sequences.
- Examples of detectable sequences include satellite DNAs and ribosomal DNA genes.
Conclusions:
- The development of hybridization in situ has a long history.
- Initial applications were constrained by the sensitivity of the technique.
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