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Fluorescence Activated Cell Sorting of Plant Protoplasts
Published on: February 18, 2010
Fluorescence-activated cell sorting according to receptor density : application for isolating transfected cell lines
1Ludwig Institute for Cancer Research, University College Branch, London, UK.
Methods in Molecular Biology (Clifton, N.J.)
|March 5, 2011
Abstract:
Isolation of cells expressing transfected cDNAcan be extremely difficult and tedious if the efficiency of expression is low (less than 1-5%). If the receptor being selected for is a cell surface or integral membrane protein at least partly exposed to the exterior, then it is possible to use the extremely powerful technique of fluorescence-activated cell sorting (FACS) to select these cells from a receptor-negative background.
