Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Hybridoma Technology

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The teat skin microbiota of organic primiparous dairy cows is dynamic during the transition period.

Preventive veterinary medicine·2025

Same author

The impact of kit, environment, and sampling contamination on the observed microbiome of bovine milk.

mSystems·2024

Same author

Exploring associations between the teat apex metagenome and <i>Staphylococcus aureus</i> intramammary infections in primiparous cows under organic directives.

Applied and environmental microbiology·2024

Same author

Bilateral vertebral arteries entering the C4 foramen transversarium with the left vertebral artery originating from the aortic arch.

Folia morphologica·2022

Same author

Investigating the cow skin and teat canal microbiomes of the bovine udder using different sampling and sequencing approaches.

Journal of dairy science·2020

Same author

The addition of ticarcillin-clavulanic acid to INRA 96 extender for stallion semen cooling.

Equine veterinary journal. Supplement·2013

Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 3, 2026

In Vitro and In Vivo Assessment of T, B and Myeloid Cells Suppressive Activity and Humoral Responses from Transplant Recipients

In Vitro and In Vivo Assessment of T, B and Myeloid Cells Suppressive Activity and Humoral Responses from Transplant Recipients

Published on: August 12, 2017

Rat x Rat Hybridomas.

¹Institute of Cancer Research, Surrey, UK.

Methods in Molecular Biology (Clifton, N.J.)

|March 5, 2011

Summary

This summary is machine-generated.

Rat hybridomas offer superior stability and high monoclonal antibody production compared to mouse models. Their IgG subclasses effectively interact with human systems, yielding significant antibody yields from ascites, especially in nude rats.

More Related Videos

Generation of Murine Monoclonal Antibodies by Hybridoma Technology

Generation of Murine Monoclonal Antibodies by Hybridoma Technology

Published on: January 2, 2017

Related Experiment Videos

Last Updated: Jun 3, 2026

In Vitro and In Vivo Assessment of T, B and Myeloid Cells Suppressive Activity and Humoral Responses from Transplant Recipients

In Vitro and In Vivo Assessment of T, B and Myeloid Cells Suppressive Activity and Humoral Responses from Transplant Recipients

Published on: August 12, 2017

Generation of Murine Monoclonal Antibodies by Hybridoma Technology

Generation of Murine Monoclonal Antibodies by Hybridoma Technology

Published on: January 2, 2017

Area of Science:

Immunotechnology
Hybridoma Technology
Monoclonal Antibody Production

Background:

Rat hybridomas exhibit enhanced culture stability over mouse hybridomas.
Rat IgG subclasses demonstrate efficient interaction with human Fc receptors and complement systems.
Ascites production in rats yields substantial specific antibody concentrations.

Purpose of the Study:

To highlight the advantages of rat hybridomas for monoclonal antibody production.
To underscore the practical benefits of rat hybridoma technology in research and therapeutics.
To detail the high yield and purity achievable through rat ascites production.

Main Methods:

Preparation and culturing of rat × rat hybridomas.
Assessment of monoclonal antibody secretion levels.
Evaluation of rat IgG subclass interactions with human Fc receptors and complement.
Ascites production in rats, including nude rats, for antibody yield determination.

Main Results:

Rat hybridomas secrete monoclonal antibodies at high and consistent levels (≥10 µg/mL).
Rat IgG subclasses show effective binding to human Fc receptors and activation of the human complement system.
Ascites fluid from rats contains high concentrations of specific antibody (1-5 mg/mL) with yields up to 30 mL per rat.
Nude rats yield ascites with minimal endogenous immunoglobulin (<1 mg/mL).

Conclusions:

Rat hybridomas are a stable and efficient platform for producing high-affinity monoclonal antibodies.
The immunological compatibility of rat IgG with human systems makes them valuable for therapeutic applications.
Rat ascites production offers a scalable and cost-effective method for generating large quantities of specific antibodies.