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Updated: Jun 3, 2026

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

Published on: August 8, 2016

Plasmid screening using single colony lysates.

J W Dale¹, P J Greenaway

¹Department of Microbiology, University of Surrey, Guildford, Surrey.

Methods in Molecular Biology (Clifton, N.J.)

|March 5, 2011

Summary

This summary is machine-generated.

This research presents a simple, rapid method for detecting plasmids in bacterial clones and environmental samples using a single colony. The technique is effective for high copy number plasmids and offers a quick screening solution.

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Area of Science:

Molecular Biology
Microbiology

Background:

Various methods exist for screening recombinant clones and natural isolates for plasmid presence.
Existing techniques can be time-consuming or complex.

Purpose of the Study:

To describe a simple and rapid method for plasmid detection.
To provide an efficient screening technique for molecular biology and environmental microbiology.

Main Methods:

The described method is based on Sherratt's technique.
It utilizes a single bacterial colony directly from an agar plate.

Main Results:

The method is the simplest and most rapid available for plasmid screening.
It is particularly effective for high copy number plasmids.

Conclusions:

This single-colony technique offers an efficient approach to plasmid detection.
It is applicable to both recombinant clones and environmental isolates.