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Maxam-Gilbert Sequencing

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Related Experiment Video

Updated: Jun 3, 2026

Parallel High Throughput Single Molecule Kinetic Assay for Site-Specific DNA Cleavage

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Chemical cleavage (maxam and gilbert) method for DNA sequence determination.

¹Department of Microbiology, The Technical University of Denmark, Lyngby, Denmark.

Methods in Molecular Biology (Clifton, N.J.)

|March 5, 2011

Summary

DNA sequencing methods differ from protein analysis. The Maxam and Gilbert method uses partial chemical degradation, while the Sanger method is widely adopted for determining DNA sequences.

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DamID-seq: Genome-wide Mapping of Protein-DNA Interactions by High Throughput Sequencing of Adenine-methylated DNA Fragments

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Area of Science:

Molecular Biology
Genetics
Biochemistry

Background:

Protein and peptide sequencing involves sequential degradation and residue identification.
DNA sequence analysis relies on high-resolution electrophoresis of oligonucleotides.
Oligonucleotides vary in length by a single nucleotide and share a common end.

Purpose of the Study:

To describe methods for DNA sequence analysis.
To differentiate DNA sequencing from protein sequencing techniques.

Main Methods:

The Maxam and Gilbert method involves chemical modification and cleavage of end-labeled DNA fragments.
This method yields four sets of oligonucleotides of varying lengths.
The Sanger method, widely used, is also discussed.

Main Results:

The Maxam and Gilbert method is also known as the partial chemical degradation method.
The Sanger method is frequently employed for DNA sequencing.

Conclusions:

DNA sequencing employs distinct techniques compared to protein sequencing.
Both Maxam and Gilbert and Sanger methods are pivotal in DNA sequence determination.